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Detection of cultured and uncultured Burkholderia cepacia complex bacteria naturally occurring in the maize rhizosphere

TitleDetection of cultured and uncultured Burkholderia cepacia complex bacteria naturally occurring in the maize rhizosphere
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2005
AuthorsPirone, L., Chiarini L., Dalmastri Claudia, Bevivino Annamaria, and Tabacchioni Silvia
JournalEnvironmental Microbiology
Volume7
Pagination1734 - 1742
Date Published2005///
Abstract

The species composition of a Burkholderia cepacia complex population naturally occurring in the maize rhizosphere was investigated by using both culture-dependent and culture-independent methods. B. cepacia complex isolates were recovered from maize root slurry on the two selective media Pseudomonas cepacia azelaic acid tryptamine (PCAT) and trypan blue tetracycline (TB-T) and subjected to identification by a combination of restriction fragment length polymorphism (RFLP) analysis and species-specific polymerase chain reaction (PCR) tests of the recA gene. DNA extracted directly from root slurry was examined by means of nested PCR to amplify recA gene with species-specific B. cepacia complex primers and to obtain a library of PCR amplified recA genes. Using the culture-dependent method the species Burkholderia cepacia, Burkholderia cenocepacia, Burkholderia ambifaria and Burkholderia pyrrocinia were identified, whereas using the culture-independent method also the species Burkholderia vietnamiensis was detected. The latter method also allowed us to highlight a higher diversity within the B. cenocepacia species. In fact, by using the culture-independent method the species B. cenocepacia recA lineages IIIA and IIID besides B. cenocepacia recA lineage IIIB were detected. Moreover, higher heterogeneity of recA RFLP patterns was observed among clones assigned to the species B. cenocepacia than among B. cenocepacia isolates from selective media. © 2005 Society for Applied Microbiology and Blackwell Publishing Ltd.

Notes

Cited By (since 1996): 10Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-27444440742&partnerID=40&md5=f533c1c7b54fb59b5014c953fa461f39
Citation Key360