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Robust RNA silencing-mediated resistance to Plum pox virus under variable abiotic and biotic conditions

TitleRobust RNA silencing-mediated resistance to Plum pox virus under variable abiotic and biotic conditions
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2014
AuthorsDi Nicola, E., Tavazza Mario, Lucioli Alessandra, Salandri L., and Ilardi V.
JournalMolecular Plant Pathology
Date Published2014
KeywordsArtichoke mottled crinkle virus (AMCV) p19, Cucumber mosaic virus (CMV) 2b, Helper component proteinase (HC-Pro), Plum pox virus (PPV)-C, Potato virus Y (PVY), RNA silencing suppressors, Temperature
Abstract

Some abiotic and biotic conditions are known to have a negative impact on post-transcriptional gene silencing (PTGS), thus representing a potential concern for the production of stable engineered virus resistance traits. However, depending on the strategy followed to achieve PTGS of the transgene, different responses to external conditions can be expected. In the present study, we utilized the Nicotiana benthamiana-Plum pox virus (PPV) pathosystem to evaluate in detail the stability of intron-hairpin (ihp)-mediated virus resistance under conditions known to adversely affect PTGS. The ihp plants grown at low or high temperatures were fully resistant to multiple PPV challenges, different PPV inoculum concentrations and even to a PPV isolate differing from the ihp construct by more than 28% at the nucleotide level. In addition, infections of ihp plants with viruses belonging to Cucumovirus, Potyvirus or Tombusvirus, all known to affect PTGS at different steps, were not able to defeat PPV resistance. Low temperatures did not affect the accumulation of transgenic small interfering RNAs (siRNAs), whereas a clear increase in the amount of siRNAs was observed during infections sustained by Cucumber mosaic virus and Potato virus Y. Our results show that the above stress factors do not represent an important concern for the production, through ihp-PTGS technology, of transgenic plants having robust virus resistance traits. © 2014 BSPP AND JOHN WILEY & SONS LTD.

Notes

Export Date: 16 September 2014Article in Press

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84899044227&partnerID=40&md5=868260f4f9683e8dbac449e589da3427
Citation Key4749